A Flexible Absorbance Proliferation, Viability and Functional Assay for Multiple Cell Types.
Uses of STEMLight™-96 Research
An alternative to STEMFluor™-96 Research and STEMGlo™-96 Research using an absorbance plate reader.
A proliferation, viability, cell functionality and cell number assays for numerous cell types derived from multiple organs and tissues.
Determine self-renewal and expansion ability and potential.
Studies on the cell proliferation process.
Determination of cells into differentiated cells.
Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) cells and cell lines.
Comparison of ES- or iPS-derived stem cell response with primary stem cells.
Benefits of using STEMLight™-96 Research
Uses a absorbance plate reader or multiparameter plate reader, available in most laboratories.
Non-subjective, instrument-based and quantitative readout procedure.
Available for adherent or non-adherent cells.
Multiplexes with flow cytometric assays.
Multiplexes with gene expression assays etc for more information from a single sample.
Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
High throughput capability.
Fast to learn and easy to use.
STEMGlo™-96 Research can be used to study virtually any cell type with proliferation capability or high intracellular ATP concentrations. These include, but are not limited to cells from the following organs and tissues:
Other cell types that can be studies using STEMGlo™-96 Research include:
Proliferating cell lines
Embryonic stem (ES) cells
Induced puripotent stem (iPS) cells
ES-or iPS-derived cells
Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
No solubilization step as in a MTT reaction. Replaces MTT assays.
Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
For Research Use Only. Not for clinical diagnostic use.
Major Equipment Required
Absorbance plate reader or multiparameter plate reader with a 490nm filter.
Sterile, 96-well plates
Non-sterile, 96-well plate for background determination
Sterile, adhesive foil covers to maintain sterility of unused wells
STEMGlo™-PREP: to detect self-renewal and expansion potential of primary stem cells and stem cell lines
HALO®-96 Research: bioluminomics™ assays for human and animal lympho-hematopoietic stem cells
HALO®-96 PREP: to detect self-renewal and expansion potential of lympho-hematopoietic stem cells
HemoFLUOR™-96: fluorescence assays for lympho-hematopoietic stem cells
HemoLIGHT™-96: absorbance assays for lympho-hematopoietic stem cells
MSCGlo™-96: bioluminomics™ assays for mesenchymal stem cells
MSCFluor™-96: fluorescence assays for mesenchymal stem cells
MSCLight™-96: absorbance assays for mesenchymal stem cells
Visit our Technical Guide Video Page
2014 Cellular Therapy & Regnerative Medicine Brochure
Proliferation & Differentiation
Toxicity to Primary Cell Systems and Cell Lines
Comparative Toxicity Testing
Stem Cells Reseacrhing
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