Ensaio de Genotoxicidade in vitro por citometria de fluxo
In Vitro MicroFlow® Kits - Miltenyi Biotec
Preclinical safety assessments often include the in vitro micronucleus assay in order to evaluate chemicals' potential to cause DNA damage. This particular assay provides information on chromosomal damage in cultured cells by detecting the formation of small membrane-bound DNA fragments (micronuclei) in the cytoplasm of interphase cells. Traditionally, the in vitro micronucleus assay requires a microscopic analysis of the treated cells which precludes its use as a high-throughput screening tool.
The In Vitro MicroFlow® Kit (Litron Laboratories) in combination with the MACSQuant® Analyzer 10 provide a fast, standardized, and automated flow cytometry–based workflow that overcomes the limitations of microscopic analysis by providing high-content information which is both reproducible and reliable.
The In Vitro MicroFlow Kits are standardized kits that enable flow cytometric detection of micronuclei derived from both adherent and suspension-cultured cells. It allows for the sequential staining and liberation of nuclei and micronuclei from each sample, prior to the analysis on a flow cytometer. When conducted following proper procedures, results from the In Vitro MicroFlow Kits are suitable for regulatory safety submissions.
Indústria Farmacêutica, Química, Alimentos
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